DNA from a black hair caught on a barbed wire fence following a sheep attack has offered 'definitive proof' big cats are roaming the British countryside, say investigators.
The strands were sent off for testing after being recovered from a farm in Gloucestershire where there had been some "unusual predatory" activity.
Suspicion was raised when video footage of a large black animal was also captured only a few miles away from where the sample was taken.
And documentary-makers, who had been investigating sightings across the UK, say the test has now come back 'positive' and confirmed the existence of black panthers and other big cats living in the UK.
A forensic laboratory took on the species identification task and used mitochondrial DNA analysis to ascertain a 99 per cent match to a big cat species.
The findings have come to light as part of filming for an upcoming documentary - Panthera Britannia Declassified - which investigates claims of big cat sightings in the UK.
Matthew Everett, from Dragonfly Films, said: "The DNA was from hairs caught on a barbed wire fence where there had been some unusual predatory activity.
"It’s taken five years for the production team to find such evidence and film its journey from collection to analysis.
"People in Gloucestershire and Britain have described what appear to be black leopards for decades. So, a leopard DNA result from a black hair sample is unsurprising.
"This is not the first such DNA result and is unlikely to be the last.
"There is a great deal of secondary evidence for these cats, such as witness reports, but hard evidence like DNA is hard to get, so the contribution from this documentary is very helpful.
"Collecting such evidence from farmers and landowners is essential - Citizen Science like this will hopefully help us learn more about the Bagheera type big cats which may be quietly naturalising here."
People across Britain have reported sightings of what appear to be black leopards for decades.
Several sightings of a large black cat were reported last year in Gloucestershire - including reports of one on the side of the motorway and another near an airport.
The farm where the DNA sample was recovered has had two individual sheep kill incidents in five years.
During that time there have been regular sightings of cats resembling black leopards in the nearby countryside.
The previous sheep carcass at the same farm as the DNA result, was assessed for its tooth pits, which are marks on the bones from carnassial teeth of an animal that has fed on the carcass.
Tooth pit analysis conducted at the Royal Agricultural University suggests that relatively large carnivores have fed on the carcasses examined, because of the impacted bones.
Witnesses commonly describe black panthers they see as 'healthy' and 'confident', which suggests they grew up in their environment.
These cats mainly prey on wild animals like deer, and they have been reported pursuing foxes and even mousing, and going for ducks, geese and pigeons. While they can switch to sheep, it seems that they much prefer natural game.
Dragonfly Films is currently pursuing broadcast options for Panthera Britannia Declassified.
An earlier version of the film “Panthera Britannia” available on Amazon Prime, Vimeo and Apple TV.
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