Gloucestershire Police, Fire and Rescue Service and County Council are urging drivers to be careful as heavy rain is anticipated across the county for a second day.
Gloucestershire Highways has already put additional response crews in place to respond to incidents as they arise.
GCC highways manager Jason Humm said: “We are fully prepared to respond to incidents as they occur, but we would urge drivers particularly to take special care before venturing out, and driving carefully and responsibly.”
The heaviest of the rainfall is anticipated between 3pm and 8pm. Commuters are advised to make their journey earlier or later to avoid the rush hours if at all possible.
Drivers are also being advised to listen to local radio bulletins for road closures, leave extra time for journeys and make sure they carry enough fuel, a fully charged mobile phone and food and water.
Superintendent Neil Mantle of Gloucestershire Police said: “Yesterday we had more than 100 calls regarding the weather, some of which were reporting incidents but many were drivers asking whether a particular road was closed and we would like to keep our lines free for those in need of help and assistance.”
Geoff Sallis, Deputy Chief Fire Officer, said: “Gloucestershire Fire & Rescue Service has planned for and is ready to respond to any incidents that occur during the anticipated bad weather this afternoon and evening. We have also added extra capacity to deal with both weather and any other incidents.”
Local residents are asked to call the Environment Agency Floodline number on 0845 988 1188 or check the website for more information about what to do if they think their homes are going to flood.
Anyone experiencing problems on the roads should contact Gloucestershire Highways on 0800 514 514 or visit the website www.highways.gov.uk/traffic-information.
Follow @GlosHighways @GlosCC @GlosFire and @Glos_Police on Twitter for updates.
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